Binding Patterns of Rotavirus Genotypes P[4], P[6], and P[8] in China with Histo-Blood Group Antigens

Xin Ma; Dan-di Li; Xiao-Man Sun; Yan-Qing Guo; Jing-Yao Xiang; Wei-Huan Wang; Li-Xia Zhang; Qing-Jiu Gu; Zhao-Jun Duan

doi:10.1371/journal.pone.0134584

Binding Patterns of Rotavirus Genotypes P[4], P[6], and P[8] in China with Histo-Blood Group Antigens

PLoS One. 2015 Aug 14;10(8):e0134584. doi: 10.1371/journal.pone.0134584. eCollection 2015.

Authors

Xin Ma¹, Dan-di Li², Xiao-Man Sun², Yan-Qing Guo², Jing-Yao Xiang², Wei-Huan Wang³, Li-Xia Zhang⁴, Qing-Jiu Gu⁴, Zhao-Jun Duan²

Affiliations

¹ China Railway Construction Corporation, Beijing Tiejian Hospital, Beijing, 100039, China; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, 102206, China.
² National Institute for Viral Disease Control and Prevention, China CDC, Beijing, 102206, China.
³ Beijing Railway Center for Disease Control and Prevention, Beijing, 100038, China.
⁴ China Railway Construction Corporation, Beijing Tiejian Hospital, Beijing, 100039, China.

Abstract

Rotaviruses (RVs) are an important cause of severe gastroenteritis in children. It has been found that RV may recognize the histo-blood group antigens (HBGAs) as ligands or receptors and bind HBGAs in a type-dependent manner. In this study, we investigated the binding specificity of VP8* proteins from human rotaviruses (RV) that are prevalent in China including genotypes P[4], P[6], and P[8]. Through the saliva- and oligosaccharide-based binding assays, we found that the VP8* proteins of P[4] and P[8] RV showed similar reactivity with the Leb and H type 1 antigens, while P[6] RV weakly bound the Leb antigen. These findings may facilitate further research into RV host specificity and vaccine development.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Animals
Antigens, Viral / genetics
Antigens, Viral / metabolism*
Blood Group Antigens / genetics
Blood Group Antigens / metabolism*
Child
Female
Gastroenteritis / blood
Gastroenteritis / virology
Genotype
Humans
Molecular Sequence Data
Protein Binding
RNA-Binding Proteins / metabolism
Rabbits
Rotavirus / genetics*
Rotavirus / immunology
Rotavirus / metabolism*
Rotavirus Infections / blood
Saliva / metabolism
Saliva / virology
Viral Nonstructural Proteins / metabolism

Substances

Antigens, Viral
Blood Group Antigens
RNA-Binding Proteins
Viral Nonstructural Proteins
NS35 protein, rotavirus

Associated data

GENBANK/KT162984
GENBANK/KT162985
GENBANK/KT162986
GENBANK/KT162987
GENBANK/KT162988

Grants and funding

The research was supported by the National Nature Science Foundation of China (NSFC Grant no. 81472003). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.