A poly(ADP-ribose) polymerase-1 activity assay based on the FRET between a cationic conjugated polymer and supercharged green fluorescent protein

Shiyun Tang; Zhou Nie; Wang Li; Daiqi Li; Yan Huang; Shouzhuo Yao

doi:10.1039/c5cc04170h

A poly(ADP-ribose) polymerase-1 activity assay based on the FRET between a cationic conjugated polymer and supercharged green fluorescent protein

Chem Commun (Camb). 2015 Oct 1;51(76):14389-92. doi: 10.1039/c5cc04170h. Epub 2015 Aug 14.

Authors

Shiyun Tang¹, Zhou Nie, Wang Li, Daiqi Li, Yan Huang, Shouzhuo Yao

Affiliation

¹ State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China. niezhou.hnu@gmail.com wli@hnu.edu.cn.

PMID: 26273706
DOI: 10.1039/c5cc04170h

Abstract

A label-free and convenient strategy for PARP-1 activity assay and inhibitors assessment has been developed based on the fluorescence resonance energy transfer (FRET) between a cationic conjugated polymer (CCP) and supercharged green fluorescent protein (scGFP).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cations / chemistry
Cations / metabolism
Drug Evaluation, Preclinical / methods
Enzyme Assays / methods*
Fluorescence Resonance Energy Transfer / methods*
Green Fluorescent Proteins / chemistry*
Green Fluorescent Proteins / metabolism
Humans
Models, Molecular
Poly (ADP-Ribose) Polymerase-1
Poly(ADP-ribose) Polymerase Inhibitors / pharmacology
Poly(ADP-ribose) Polymerases / analysis
Poly(ADP-ribose) Polymerases / blood
Poly(ADP-ribose) Polymerases / metabolism*
Poly(ADP-ribose) Polymerases / urine
Polymers / chemistry
Polymers / metabolism

Substances

Cations
Poly(ADP-ribose) Polymerase Inhibitors
Polymers
Green Fluorescent Proteins
PARP1 protein, human
Poly (ADP-Ribose) Polymerase-1
Poly(ADP-ribose) Polymerases