Improved Protocols for Illumina Sequencing

Curr Protoc Hum Genet. 2014 Jan 21:80:18.2.1-42. doi: 10.1002/0471142905.hg1802s80.

Abstract

In this unit, we describe a set of improvements that have been made to the standard Illumina protocols to make the sequencing process more reliable in a high-throughput environment, reduce amplification bias, narrow the distribution of insert sizes, and reliably obtain high yields of data.

Keywords: Illumina; next-generation; protocols; sequencer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • High-Throughput Nucleotide Sequencing / methods*
  • High-Throughput Nucleotide Sequencing / standards
  • Nucleic Acid Denaturation