Evaluation of chemokines CXCL8 and CCL2, serotonin, and vascular endothelial growth factor serum concentrations in healthy dogs from seven breeds with variable predisposition for canine idiopathic pulmonary fibrosis

E Roels; E Krafft; N Antoine; F Farnir; H P Laurila; S Holopainen; M M Rajamäki; C Clercx

doi:10.1016/j.rvsc.2015.05.020

Evaluation of chemokines CXCL8 and CCL2, serotonin, and vascular endothelial growth factor serum concentrations in healthy dogs from seven breeds with variable predisposition for canine idiopathic pulmonary fibrosis

Res Vet Sci. 2015 Aug:101:57-62. doi: 10.1016/j.rvsc.2015.05.020. Epub 2015 Jun 5.

Authors

E Roels¹, E Krafft², N Antoine³, F Farnir⁴, H P Laurila⁵, S Holopainen⁵, M M Rajamäki⁵, C Clercx²

Affiliations

¹ Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liege, Bd de Colonster, Liege, Belgium. Electronic address: roels@ulg.ac.be.
² Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liege, Bd de Colonster, Liege, Belgium.
³ Department of Morphology and Pathology, Histology, Faculty of Veterinary Medicine, University of Liege, Bd de Colonster, Liege, Belgium.
⁴ Department of Animal Productions, Biostatistics and Bioinformatics Applied to Veterinary Sciences, Faculty of Veterinary Medicine, University of Liege, Bd de Colonster, Liege, Belgium.
⁵ Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, PO Box 57, 00014 University of Helsinki, Helsinki, Finland.

PMID: 26267090
DOI: 10.1016/j.rvsc.2015.05.020

Abstract

The West Highland white terrier (WHWT) is particularly prone to canine idiopathic pulmonary fibrosis (CIPF). We hypothesized that higher circulating concentrations of chemokines CXCL8, CCL2, serotonin (5-HT), or vascular endothelial growth factor (VEGF) could serve as predisposing factors for CIPF development in the WHWT breed. Serum samples from 103 healthy dogs of seven different breeds variably predisposed to CIPF were collected. Serum CXCL8 concentrations were higher in healthy WHWT compared with each of the other groups of healthy dogs. Serum CCL2 concentrations were higher in healthy WHWT and Maltese compared with King Charles spaniels and Malinois Belgian shepherds. No relevant inter-breed differences were observed for serum 5-HT concentrations regarding CIPF predisposition. VEGF values from 89.3% of samples tested were below the kit detection limit. In conclusion, high CXCL8 blood concentrations and possibly CCL2 concentrations might be related to the breed predisposition of the WHWT for CIPF and warrants further investigations.

Keywords: CCL2; CXCL8; Canine idiopathic pulmonary fibrosis; Serotonin; VEGF.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chemokine CCL2 / blood*
Dog Diseases / blood*
Dog Diseases / genetics
Dogs
Genetic Predisposition to Disease / genetics*
Genotype
Idiopathic Pulmonary Fibrosis / blood
Idiopathic Pulmonary Fibrosis / genetics
Idiopathic Pulmonary Fibrosis / veterinary*
Interleukin-8 / blood*
Serotonin / blood*
Species Specificity
Vascular Endothelial Growth Factor A / blood*

Substances

Chemokine CCL2
Interleukin-8
Vascular Endothelial Growth Factor A
Serotonin