Abstract
The inhibitory effect of three chromones 1-3 and two coumarins 4-5 on the production of nitric oxide (NO) was evaluated in LPS-induced RAW 264.7 macrophage cells. Among the compounds tested heterocarpin (1), a furochromone, significantly inhibited its production in a dose-dependent manner. In addition, heterocarpin suppressed prostaglandin E2 (PGE2) production and expression of cytokines such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
Keywords:
Corydalis heterocarpa; anti-inflammatory effect; furochromone; heterocarpin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents, Non-Steroidal / isolation & purification
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
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Chromones / chemistry
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Chromones / pharmacology*
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Corydalis / chemistry*
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Coumarins / chemistry
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Coumarins / pharmacology*
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Cyclooxygenase 2 / biosynthesis
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Cyclooxygenase 2 Inhibitors / pharmacology
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Cytokines / antagonists & inhibitors
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Cytokines / biosynthesis
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Dinoprostone / antagonists & inhibitors
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Dinoprostone / biosynthesis
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Dose-Response Relationship, Drug
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Lipopolysaccharides / pharmacology
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Macrophages / drug effects*
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Macrophages / metabolism
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Mice
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Nitric Oxide / antagonists & inhibitors
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Nitric Oxide / biosynthesis
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Nitric Oxide Synthase Type II / antagonists & inhibitors
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Nitric Oxide Synthase Type II / biosynthesis
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RAW 264.7 Cells
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Signal Transduction / drug effects
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Chromones
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Coumarins
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Cyclooxygenase 2 Inhibitors
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Cytokines
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Lipopolysaccharides
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Nitric Oxide
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Nitric Oxide Synthase Type II
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Cyclooxygenase 2
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Dinoprostone