Diterpene Constituents of Euphorbia exigua L. and Multidrug Resistance Reversing Activity of the Isolated Diterpenes

Dóra Rédei; Klára Boros; Peter Forgo; Joseph Molnár; Zoltán Kele; István Pálinkó; Gyula Pinke; Judit Hohmann

doi:10.1002/cbdv.201400260

Diterpene Constituents of Euphorbia exigua L. and Multidrug Resistance Reversing Activity of the Isolated Diterpenes

Chem Biodivers. 2015 Aug;12(8):1214-21. doi: 10.1002/cbdv.201400260.

Authors

Dóra Rédei¹, Klára Boros², Peter Forgo², Joseph Molnár³, Zoltán Kele⁴, István Pálinkó⁵, Gyula Pinke⁶, Judit Hohmann²

Affiliations

¹ Department of Pharmacognosy, University of Szeged, Eötvös u. 6, HU-6720 Szeged, (phone: +36 62 546451, fax: +36 62 545704). redei@pharm.u-szeged.hu.
² Department of Pharmacognosy, University of Szeged, Eötvös u. 6, HU-6720 Szeged, (phone: +36 62 546451, fax: +36 62 545704).
³ Department of Medical Microbiology and Immunobiology, University of Szeged, Dóm tér 10, HU-6720 Szeged.
⁴ Department of Medical Chemistry, University of Szeged, Dóm tér 8, HU-6720 Szeged.
⁵ Department of Organic Chemistry, University of Szeged, Dóm tér 8, HU-6720 Szeged.
⁶ Department of Botany, Faculty of Agricultural and Food Sciences, University of West Hungary, Lucsony u. 15 - 17, HU-9200 Mosonmagyaróvár.

PMID: 26265573
DOI: 10.1002/cbdv.201400260

Abstract

Phytochemical investigation of the MeOH extract obtained from the aerial parts of the annual weed Euphorbia exigua L. resulted in the isolation of two novel (1, 2) and one known (3) jatrophane diterpenes. Their structures were established by extensive 1D- and 2D-NMR spectroscopy and HR-ESI-MS. The isolated compounds were evaluated for multidrug resistance (MDR) reversing activity on human MDR gene-transfected L5178 mouse lymphoma cells; and all three compounds were found to modulate the intracellular drug accumulation.

Keywords: Diterpene; Euphorbia exigua; Jatrophane; Multidrug resistance; P-Glycoprotein.

MeSH terms

ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism*
Animals
Cell Line, Tumor
Diterpenes / chemistry*
Diterpenes / isolation & purification
Diterpenes / pharmacology*
Drug Resistance, Multiple / drug effects*
Drug Resistance, Neoplasm / drug effects
Euphorbia / chemistry*
Humans
Lymphoma / drug therapy
Lymphoma / genetics
Lymphoma / metabolism
Mice
Transfection

Substances

ATP Binding Cassette Transporter, Subfamily B, Member 1
Diterpenes
jatrophane