Abstract
Deoxynivalenol (DON) is a mycotoxin affecting animals and plants. This toxin synthesized by Fusarium culmorum and Fusarium graminearum is currently believed to play a decisive role in the fungal phytopathogenesis as a virulence factor. Using cultured cells of Nicotiana tabacum BY2, we showed that DON-induced programmed cell death (PCD) could require transcription and translation processes, in contrast to what was observed in animal cells. DON could induce different cross-linked pathways involving (i) reactive oxygen species (ROS) generation linked, at least partly, to a mitochondrial dysfunction and a transcriptional down-regulation of the alternative oxidase (Aox1) gene and (ii) regulation of ion channel activities participating in cell shrinkage, to achieve PCD.
Keywords:
Deoxynivalenol; Fusarium; Ion channels; Nicotiana tabacum; Programmed cell death.
Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis / drug effects*
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Calcium / metabolism
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Cell Line
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Cell Membrane / drug effects
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Cell Membrane / metabolism
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Gene Expression Regulation, Plant / drug effects
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Ion Channel Gating / drug effects
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Ion Channels / metabolism
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Membrane Potentials / drug effects
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Mitochondria / drug effects
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Mitochondria / metabolism
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Mitochondrial Proteins / genetics
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Mitochondrial Proteins / metabolism
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Mycotoxins / toxicity*
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Nicotiana / cytology*
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Nicotiana / drug effects
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Nicotiana / enzymology
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Nicotiana / genetics
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Oxidoreductases / genetics
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Oxidoreductases / metabolism
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Plant Cells / drug effects
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Plant Cells / metabolism*
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Plant Proteins / genetics
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Plant Proteins / metabolism
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Reactive Oxygen Species / metabolism
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Respiratory Burst / drug effects
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Suspensions
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Trichothecenes / toxicity*
Substances
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Ion Channels
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Mitochondrial Proteins
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Mycotoxins
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Plant Proteins
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Reactive Oxygen Species
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Suspensions
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Trichothecenes
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Oxidoreductases
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alternative oxidase
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deoxynivalenol
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Calcium