Cytoprotective effects of grape seed extract on human gingival fibroblasts in relation to its antioxidant potential

Yusuke Katsuda; Yoshimi Niwano; Takuji Nakashima; Takayuki Mokudai; Keisuke Nakamura; Satomi Oizumi; Taro Kanno; Hiroyasu Kanetaka; Hiroshi Egusa

doi:10.1371/journal.pone.0134704

Cytoprotective effects of grape seed extract on human gingival fibroblasts in relation to its antioxidant potential

PLoS One. 2015 Aug 10;10(8):e0134704. doi: 10.1371/journal.pone.0134704. eCollection 2015.

Authors

Yusuke Katsuda¹, Yoshimi Niwano¹, Takuji Nakashima², Takayuki Mokudai¹, Keisuke Nakamura¹, Satomi Oizumi¹, Taro Kanno¹, Hiroyasu Kanetaka¹, Hiroshi Egusa¹

Affiliations

¹ Tohoku University Graduate School of Dentistry, Sendai, Japan.
² Kiasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo, Japan.

Abstract

Cytoprotective effects of short-term treatment with grape seed extract (GSE) upon human gingival fibroblasts (hGFs) were evaluated in relation to its antioxidant properties and compared with those of a water-soluble analog of vitamin E: trolox (Tx). GSE and Tx showed comparable antioxidant potential in vitro against di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH; a stable radical), hydroxyl radical (•OH), singlet oxygen (1O2), and hydrogen peroxide (H2O2). Pretreatment or concomitant treatment with GSE for 1 min protected hGFs from oxidative stressors, including H2O2, acid-electrolyzed water (AEW), and 1O2, and attenuated the intracellular formation of reactive oxygen species induced by H2O2 and AEW. Tx also reduced the H2O2- and AEW-induced intracellular formation of reactive oxygen species, but showed no cytoprotective effects on hGFs exposed to H2O2, AEW, or 1O2. These results suggest that the cytoprotective effects of GSE are likely exerted independently of its antioxidant potential.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antioxidants / administration & dosage*
Antioxidants / chemistry
Cell Survival / drug effects
Fibroblasts / drug effects
Gingiva / drug effects
Gingiva / metabolism*
Grape Seed Extract / administration & dosage*
Grape Seed Extract / chemistry
Humans
Hydrogen Peroxide / toxicity
Oxidative Stress / drug effects*
Reactive Oxygen Species / metabolism

Substances

Antioxidants
Grape Seed Extract
Reactive Oxygen Species
Hydrogen Peroxide

Grants and funding

This research was supported by the Ministry of Education, Culture, Sports, Science and Technology Japan, Grant-in-Aid for Scientific Research (C), 26460116, 2014. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.