Optimization of in vitro cultivation strategies for human adipocyte derived stem cells

K Storck; J Ell; S Regn; B Rittler-Ungetüm; H Mayer; T Schantz; D Müller; M Buchberger

doi:10.4161/21623945.2014.987580

Optimization of in vitro cultivation strategies for human adipocyte derived stem cells

Adipocyte. 2015 Jan 20;4(3):181-7. doi: 10.4161/21623945.2014.987580. eCollection 2015 Jul-Sep.

Authors

K Storck¹, J Ell¹, S Regn¹, B Rittler-Ungetüm¹, H Mayer¹, T Schantz², D Müller², M Buchberger¹

Affiliations

¹ Department of Oto- Rhino- Laryngology; Head and Neck Surgery; Klinikum Rechts der Isar der TU München ; Munich, Germany.
² Department of Plastic and Reconstructive Surgery; Klinikum Rechts der Isar der TU München ; Munich, Germany.

Abstract

With adipose-derived stem cells being in the focus of research in regenerative medicine, the need arises for fast reliable cultivation protocols. We have tested the cultivation of human adipose-derived stem cells in endothelial cell growth medium prior to induction and differentiation, against the long-established use of DMEM/F12 medium-based cultivation protocols. We found that cultivation in endothelial cell growth medium not only accelerates growth before induction and differentiation, but also allows shorter induction and differentiation times than those following precultivation with DMEM/F12 medium with regard to the formation of mature adipocytes and to the viability undifferentiated cells. These results were first observed morphologically but could be confirmed by performing adiponectin ELISA and cell proliferation assays.

Keywords: ADSC; ADSCs, Adipocyte Derived Stem Cells; BM, Basal medium; DM, Differentiation medium; FBS, Fetal Bovine Serum; IM, Induction medium; adipocyte; cell-culture; culture medium; differentiation; induction; preadipocyte.