Characterization and biological activities of a novel polysaccharide isolated from raspberry (Rubus idaeus L.) fruits

Abstract

A water-soluble polysaccharide namely RCP-II from raspberry fruits was obtained by complex enzyme method followed by successive purification using macroporous resin D4020 and Sephadex G-100 columns. RCP-II was an acidic heteropolysaccharide and the characteristic structure of polysaccharide was determined. The carbohydrate of RCP-II was composed with galacturonic acid, rhamnose, arabinose, xylose, glucose and galactose in a molar ratio of 1.00:0.55:1.19:0.52:0.44:1.90 and the average molecular weight was estimated to be 4013 Da, based on dextran standards. RCP-II presented high scavenging activity toward DPPH•, HO•, O2(•-) in a concentration-dependent manner. The determination of the inhibitory activity on protein glycation showed that in 14 days of incubation the inhibitory ability of RCP-II was more effective on the development of non-enzymatic glycation reaction at early phase than that at the following two phases.

Keywords: Antioxidant activity; Ascorbic acid (PubChem CID: 54670067); Carbazole (PubChem CID: 6854); DPPH (PubChem CID: 2735032); Girard-T (PubChem CID: 67156); Non-enzymatic glycation inhibition activity; Polysaccharide; Purification; Rubus idaeus L.; Trifluoroacetic acid (PubChem CID: 6422); d-Arabinose (PubChem CID: 66308); d-Galacturonic acid (PubChem CID: 84740); d-Glucose (PubChem CID: 5793).