High Resolution Melting-Typing (HRMT) of methicillin-resistant Staphylococcus aureus (MRSA): The new frontier to replace multi-locus sequence typing (MLST) for epidemiological surveillance studies

Gino Mongelli; Dafne Bongiorno; Marilena Agosta; Sabrina Benvenuto; Stefania Stefani; Floriana Campanile

doi:10.1016/j.mimet.2015.08.001

High Resolution Melting-Typing (HRMT) of methicillin-resistant Staphylococcus aureus (MRSA): The new frontier to replace multi-locus sequence typing (MLST) for epidemiological surveillance studies

J Microbiol Methods. 2015 Oct:117:136-8. doi: 10.1016/j.mimet.2015.08.001. Epub 2015 Aug 5.

Authors

Gino Mongelli¹, Dafne Bongiorno¹, Marilena Agosta¹, Sabrina Benvenuto¹, Stefania Stefani¹, Floriana Campanile²

Affiliations

¹ Medical Molecular Microbiology and Antibiotic Resistance laboratory (MMAR Lab), Department of Biomedical and Biotechnological Sciences, University of Catania, Italy.
² Medical Molecular Microbiology and Antibiotic Resistance laboratory (MMAR Lab), Department of Biomedical and Biotechnological Sciences, University of Catania, Italy. Electronic address: f.campanile@unict.it.

PMID: 26255133
DOI: 10.1016/j.mimet.2015.08.001

Abstract

We report an implemented molecular-typing-method based on HRMA to detect SNPs within MLST loci, characterizing 100 clinical MRSA and 11 control strains, representative of Italian clones. The results provide solid evidence that HRMT could be a fast, cost-effective and reliable alternative to MLST, for MRSA molecular epidemiology.

Keywords: Cost and time reduction; High Resolution Melting Typing (HRMT); Methicillin-resistant Staphylococcus aureus (MRSA); Molecular-epidemiology; Single-nucleotide polymorphisms (SNPs).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
Methicillin-Resistant Staphylococcus aureus / genetics*
Molecular Epidemiology / methods*
Molecular Typing / methods*
Multilocus Sequence Typing
Polymorphism, Single Nucleotide / genetics
Staphylococcal Infections / epidemiology
Staphylococcal Infections / microbiology*