Crystallographic analysis of FAD-dependent glucose dehydrogenase

Acta Crystallogr F Struct Biol Commun. 2015 Aug;71(Pt 8):1017-9. doi: 10.1107/S2053230X15010742. Epub 2015 Jul 28.

Abstract

An FAD-dependent glucose dehydrogenase (GDH) from Aspergillus terreus was purified and crystallized at 293 K using the sitting-drop vapour-diffusion method. A data set was collected to a resolution of 1.6 Å from a single crystal at 100 K using a rotating-anode X-ray source. The crystal belonged to space group P21, with unit-cell parameters a = 56.56, b = 135.74, c = 74.13 Å, β = 90.37°. The asymmetric unit contained two molecules of GDH. The Matthews coefficient was calculated to be 2.2 Å(3) Da(-1) and the solvent content was estimated to be 44%.

Keywords: FAD; glucose dehydrogenase; glucose sensor.

MeSH terms

  • Amino Acid Sequence
  • Aspergillus / chemistry*
  • Aspergillus / enzymology
  • Aspergillus oryzae / genetics
  • Aspergillus oryzae / metabolism
  • Cloning, Molecular
  • Crystallization
  • Crystallography, X-Ray
  • Flavin-Adenine Dinucleotide / chemistry*
  • Fungal Proteins / chemistry*
  • Fungal Proteins / genetics
  • Gene Expression
  • Glucose 1-Dehydrogenase / chemistry*
  • Glucose 1-Dehydrogenase / genetics
  • Molecular Sequence Data
  • Plasmids / chemistry
  • Plasmids / metabolism
  • Protein Multimerization
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Sequence Alignment

Substances

  • Fungal Proteins
  • Recombinant Proteins
  • Flavin-Adenine Dinucleotide
  • Glucose 1-Dehydrogenase