Antimicrobial efficacy of green synthesized drug blended silver nanoparticles against dental caries and periodontal disease causing microorganisms

R Emmanuel; Selvakumar Palanisamy; Shen-Ming Chen; K Chelladurai; S Padmavathy; M Saravanan; P Prakash; M Ajmal Ali; Fahad M A Al-Hemaid

doi:10.1016/j.msec.2015.06.033

Antimicrobial efficacy of green synthesized drug blended silver nanoparticles against dental caries and periodontal disease causing microorganisms

Mater Sci Eng C Mater Biol Appl. 2015 Nov 1:56:374-9. doi: 10.1016/j.msec.2015.06.033. Epub 2015 Jun 17.

Authors

R Emmanuel¹, Selvakumar Palanisamy², Shen-Ming Chen³, K Chelladurai², S Padmavathy⁴, M Saravanan⁵, P Prakash⁶, M Ajmal Ali⁷, Fahad M A Al-Hemaid⁷

Affiliations

¹ Post Graduate & Research Department of Chemistry, Thiagarajar College, Madurai 625009, Tamilnadu, India.
² Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei 106, Taiwan, ROC.
³ Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei 106, Taiwan, ROC. Electronic address: smchen78@ms15.hinet.net.
⁴ Department of Zoology and Microbiology, Thiagarajar College, Madurai 625009, Tamilnadu, India.
⁵ Institute of Biomedical Sciences, College of Health Science, Mekelle University, Mekelle 1871, Ethiopia.
⁶ Post Graduate & Research Department of Chemistry, Thiagarajar College, Madurai 625009, Tamilnadu, India. Electronic address: kmpprakash@gmail.com.
⁷ Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.

PMID: 26249603
DOI: 10.1016/j.msec.2015.06.033

Abstract

Development of biologically inspired green synthesis of silver nanoparticles is evolving into an important branch of nano-biotechnology. In the present investigation, we report the green synthesis of silver nanoparticles (AgNPs) employing the leaf extract of Justicia glauca. Water-soluble organics present in the leaf extract are mainly responsible for the reduction of silver nitrate (AgNO3) solution to AgNPs. The AgNPs are 10-20nm in dimensions as determined by TEM images. The antimicrobial activities of green synthesized AgNPs and drug blended AgNPs have been evaluated against the dental caries and periodontal disease causing microorganisms such as Streptococcus mutans, Staphylococcus aureus, Lactobacillus acidophilus, Micrococcus luteus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. The AgNPs and drug blended AgNPs show a significant antibacterial and antifungal activity. Minimum inhibitory concentration (MIC) value of AgNPs determined against the selected dental caries and periodontal disease causing microorganisms are noticeable between the range of 25-75μg/mL.

Keywords: Antimicrobial activities; Dental caries; Justicia glauca; Periodontal disease; Silver nanoparticles.

MeSH terms

Anti-Bacterial Agents* / chemical synthesis
Anti-Bacterial Agents* / chemistry
Anti-Bacterial Agents* / pharmacology
Bacteria / growth & development
Candida albicans / growth & development
Dental Caries* / drug therapy
Dental Caries* / microbiology
Justicia / chemistry*
Metal Nanoparticles / chemistry*
Periodontitis* / drug therapy
Periodontitis* / microbiology
Plant Extracts / chemistry
Plant Leaves / chemistry
Silver / chemistry*

Substances

Anti-Bacterial Agents
Plant Extracts
Silver