Proliferation and osteogenic differentiation of mesenchymal stromal cells in a novel porous hydroxyapatite scaffold

Regen Med. 2015;10(5):579-90. doi: 10.2217/rme.15.27.

Abstract

Aim: To compare the effect of bovine bone derived porous hydroxyapatite (BDHA) scaffold on proliferation and osteogenic differentiation of human bone marrow-derived mesenchymal stromal cells (hMSCs) compared with commercial hydroxyapatite (CHA) scaffold.

Materials and methods: The porosity and pore size were analyzed using micro-CT. The biocompatibility was demonstrated by alamar blue assay, and cell attachment through SEM and Hoechst staining. The osteogenic differentiation was demonstrated using biochemical assay and osteogenic gene expression.

Results: BDHA and CHA scaffolds showed porosity of 76.6 ± 0.6 and 64.3 ± 0.3% and pore size diameter of 0.04-0.25 and 0.1-2.6 mm, respectively. hMSCs proliferation, ALP activity, osteocalcin secretion and osteogenic gene expression are comparable in both the scaffolds.

Conclusion: These results demonstrated that BDHA is biocompatible, supports cell adhesion and promotes proliferation and osteogenic differentiation.

Keywords: hydroxyapatite; mesenchymal stromal cell; osteogenic differentiation; tissue engineering.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biocompatible Materials / chemistry
  • Bone and Bones / pathology
  • Cattle
  • Cell Adhesion
  • Cell Differentiation
  • Cell Proliferation
  • Durapatite / chemistry
  • Humans
  • Mesenchymal Stem Cells / cytology*
  • Microscopy, Confocal
  • Microscopy, Electron, Scanning
  • Osteocalcin / metabolism
  • Osteogenesis / physiology*
  • Porosity
  • Tissue Scaffolds*
  • Tomography, X-Ray Computed
  • X-Ray Microtomography

Substances

  • Biocompatible Materials
  • Osteocalcin
  • Durapatite