Tackling Cell Transplantation Anoikis: An Injectable, Shape Memory Cryogel Microcarrier Platform Material for Stem Cell and Neuronal Cell Growth

Small. 2015 Oct;11(38):5047-53. doi: 10.1002/smll.201500898. Epub 2015 Aug 3.

Abstract

Highly macroporous semisynthetic cryogel microcarriers can be synthesized for culturing stem cells and neuronal type cells. Growth factors loaded to heparin-containing microcarriers show near zero-order release kinetics and cell-loaded microcarriers can be injected through a fine gauge cannula without negative effect on the cells. These carriers can be applied for cell transplantation applications.

Keywords: adherent matrix; biomaterials; cryogels; heparin; microcarriers; neurotrophin; shape memory; star-PEG.

MeSH terms

  • Animals
  • Anoikis / drug effects*
  • Cell Movement / drug effects
  • Cell Proliferation / drug effects
  • Cell Transplantation*
  • Cryogels / pharmacology*
  • Injections
  • Microspheres*
  • Neurons / cytology*
  • Neurons / drug effects
  • PC12 Cells
  • Rats
  • Rats, Transgenic
  • Stem Cells / cytology*
  • Stem Cells / drug effects

Substances

  • Cryogels