Clonogrouping, a Rapid Multiplex PCR Method for Identification of Major Clones of Listeria monocytogenes

Viviane Chenal-Francisque; Mylène M Maury; Morgane Lavina; Marie Touchon; Alexandre Leclercq; Marc Lecuit; Sylvain Brisse

doi:10.1128/JCM.00738-15

Clonogrouping, a Rapid Multiplex PCR Method for Identification of Major Clones of Listeria monocytogenes

J Clin Microbiol. 2015 Oct;53(10):3355-8. doi: 10.1128/JCM.00738-15. Epub 2015 Jul 29.

Authors

Viviane Chenal-Francisque¹, Mylène M Maury², Morgane Lavina¹, Marie Touchon³, Alexandre Leclercq¹, Marc Lecuit⁴, Sylvain Brisse⁵

Affiliations

¹ Institut Pasteur, Biology of Infection Unit, National Reference Centre and WHO Collaborating Centre for Listeria, Paris, France.
² Institut Pasteur, Microbial Evolutionary Genomics, Paris, France CNRS, UMR 3525, Paris, France Paris Diderot University, Sorbonne Paris Cité, Cellule Pasteur, Paris, France.
³ Institut Pasteur, Microbial Evolutionary Genomics, Paris, France CNRS, UMR 3525, Paris, France.
⁴ Institut Pasteur, Biology of Infection Unit, National Reference Centre and WHO Collaborating Centre for Listeria, Paris, France INSERM Unit 1117, Paris, France Paris Descartes University, Sorbonne Paris Cité, Institut Imagine, Necker-Enfants Malades University Hospital, Division of Infectious Diseases and Tropical Medicine, APHP, Paris, France sylvain.brisse@pasteur.fr marc.lecuit@pasteur.fr.
⁵ Institut Pasteur, Microbial Evolutionary Genomics, Paris, France CNRS, UMR 3525, Paris, France sylvain.brisse@pasteur.fr marc.lecuit@pasteur.fr.

Abstract

Three multiplex PCR assays were developed to identify the 11 most common Listeria monocytogenes clones in clinical and food samples; 270 (95.7%) of 282 strains of serogroups IVb, IIb, IIa, and IIc were identified accurately. This novel tool is a rapid and efficient alternative to multilocus sequence typing for identification of L. monocytogenes clones.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Food Microbiology
Genotype*
Humans
Listeria monocytogenes / classification*
Listeria monocytogenes / genetics*
Listeria monocytogenes / isolation & purification
Listeriosis / diagnosis
Listeriosis / microbiology
Molecular Typing / methods*
Multiplex Polymerase Chain Reaction / methods*
Time Factors