Abstract
Endothelial progenitor cells (EPCs) are being investigated for advanced therapies, and matrix metalloproteinase 9 (MMP9) has an important role in stroke recovery. Our aim was to determine whether tissue MMP9 influences the EPC-induced angiogenesis after ischemia. Wild-type (WT) and MMP9-deficient mice (MMP9/KO) were subjected to cerebral ischemia and treated with vehicle or outgrowth EPCs. After 3 weeks, we observed an increase in the peri-infarct vessel density in WT animals but not in MMP9/KO mice; no differences were found in the vehicle-treated groups. Our data suggest that tissue MMP9 has a crucial role in EPC-induced vascular remodeling after stroke.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Brain Ischemia / genetics*
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Brain Ischemia / pathology
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Brain Ischemia / therapy
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Cerebral Cortex / pathology*
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Cerebral Infarction / pathology
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Cerebral Infarction / physiopathology
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Cerebral Veins / growth & development
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Cerebral Veins / physiology*
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DNA-Binding Proteins
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Doublecortin Domain Proteins
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Endothelial Progenitor Cells*
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Magnetic Resonance Imaging
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Male
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Matrix Metalloproteinase 9 / deficiency
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Matrix Metalloproteinase 9 / genetics
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Matrix Metalloproteinase 9 / physiology*
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Mice
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Mice, Knockout
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Microtubule-Associated Proteins / genetics
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Microtubule-Associated Proteins / physiology
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Neovascularization, Physiologic / genetics
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Neovascularization, Physiologic / physiology
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / physiology
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Neuropeptides / genetics
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Neuropeptides / physiology
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Nuclear Proteins / genetics
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Nuclear Proteins / physiology
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Stem Cell Transplantation / methods*
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Stroke / therapy
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Vascular Remodeling*
Substances
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DNA-Binding Proteins
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Doublecortin Domain Proteins
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Microtubule-Associated Proteins
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Nerve Tissue Proteins
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NeuN protein, mouse
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Neuropeptides
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Nuclear Proteins
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Matrix Metalloproteinase 9