In silico analyses of dystrophin Dp40 cellular distribution, nuclear export signals and structure modeling

Alejandro Martínez-Herrera; Jorge Aragón; Rosa Ma Bermúdez-Cruz; Ma Luisa Bazán; Gabriela Soid-Raggi; Víctor Ceja; Andrea Santos Coy-Arechavaleta; Víctor Alemán; Francisco Depardón; Cecilia Montañez

doi:10.1016/j.dib.2015.06.007

In silico analyses of dystrophin Dp40 cellular distribution, nuclear export signals and structure modeling

Data Brief. 2015 Jun 25:4:349-52. doi: 10.1016/j.dib.2015.06.007. eCollection 2015 Sep.

Affiliations

¹ Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, México, D. F., Mexico.
² Departamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, México, D. F., Mexico.

Abstract

Dystrophin Dp40 is the shortest protein encoded by the DMD (Duchenne muscular dystrophy) gene. This protein is unique since it lacks the C-terminal end of dystrophins. In this data article, we describe the subcellular localization, nuclear export signals and the three-dimensional structure modeling of putative Dp40 proteins using bioinformatics tools. The Dp40 wild type protein was predicted as a cytoplasmic protein while the Dp40n4 was predicted to be nuclear. Changes L93P and L170P are involved in the nuclear localization of Dp40n4 protein. A close analysis of Dp40 protein scored that amino acids (93)LEQEHNNLV(101) and (168)LLLHDSIQI(176) could function as NES sequences and the scores are lost in Dp40n4. In addition, the changes L93/170P modify the tertiary structure of putative Dp40 mutants. The analysis showed that changes of residues 93 and 170 from leucine to proline allow the nuclear localization of Dp40 proteins. The data described here are related to the research article entitled "EF-hand domains are involved in the differential cellular distribution of dystrophin Dp40" (J. Aragón et al. Neurosci. Lett. 600 (2015) 115-120) [1].

Keywords: Cellular distribution; Dystrophin Dp40; In silico.