Datasets from label-free quantitative proteomic analysis of human glomeruli with sclerotic lesions

Ying Zhang; Bo Xu; Naohiko Kinoshita; Yutaka Yoshida; Masayuki Tasaki; Hidehiko Fujinaka; Sameh Magdeldin; Eishin Yaoita; Tadashi Yamamoto

doi:10.1016/j.dib.2015.05.013

Datasets from label-free quantitative proteomic analysis of human glomeruli with sclerotic lesions

Data Brief. 2015 May 27:4:180-5. doi: 10.1016/j.dib.2015.05.013. eCollection 2015 Sep.

Authors

Ying Zhang¹, Bo Xu², Naohiko Kinoshita², Yutaka Yoshida², Masayuki Tasaki³, Hidehiko Fujinaka⁴, Sameh Magdeldin⁵, Eishin Yaoita², Tadashi Yamamoto¹

Affiliations

¹ Department of Structural Pathology, Institute of Nephrology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan ; Biofluid Biomarker Center (BB-C), Institute for Research Collaboration and Promotion, Niigata University, Niigata, Japan.
² Department of Structural Pathology, Institute of Nephrology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan.
³ Division of Urology, Department of Regenerative and Transplant Medicine, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan.
⁴ Department of Structural Pathology, Institute of Nephrology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan ; Institute of Clinical Research, Niigata National Hospital, Kashiwazaki, Japan.
⁵ Department of Structural Pathology, Institute of Nephrology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan ; Department of Physiology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.

Abstract

Human glomeruli with intermediate (i-GS) and advanced (GS) sclerotic lesions as well as the normal control (Nor) were captured from laser microdissection, digested by trypsin and subjected to shotgun LC-MS/MS analysis (LTQ-Orbitrap XL). The label-free quantification was performed using the Normalized Spectral Index (SI N ) to assess the relative molar concentration of each protein identified in a sample. All the experimental data are shown in this article. The data is associated to the research article submitted to Journal of Proteomics [1].