pI-Control in Comparative Fluorescence Gel Electrophoresis (CoFGE) using amphoteric azo dyes

Marina Hanneken; Karel Šlais; Simone König

doi:10.1016/j.dib.2015.03.007

pI-Control in Comparative Fluorescence Gel Electrophoresis (CoFGE) using amphoteric azo dyes

Data Brief. 2015 Apr 1:3:221-8. doi: 10.1016/j.dib.2015.03.007. eCollection 2015 Jun.

Authors

Marina Hanneken¹, Karel Šlais², Simone König¹

Affiliations

¹ Core Unit Proteomics, Interdisciplinary Center for Clinical Research, University of Münster, Germany.
² Institute of Analytical Chemistry of the ASCR, v. v. i., Brno, Czech Republic.

Abstract

Amphoteric azo dyes were used for internal control of pI values in Comparative two-dimensional Fluorescence Gel Electrophoresis (CoFGE) [1]. The 2D-gel images of separated Escherichia coli proteins as well as those of colored amphoteric dyes separated by isoelectric focussing are presented. The latter were used to correct for variation in the first electrophoretic dimension and further improve protein coordinate assignment in 2D-gel electrophoresis. Data tables are supplied to demonstrate pI-value calibration and the effect on the assignment of protein spot coordinates.

Keywords: 2D-PAGE; CoFGE; Gel electrophoresis; pI.