KAEA (SUDPRO), a member of the ubiquitous KEOPS/EKC protein complex, regulates the arginine catabolic pathway and the expression of several other genes in Aspergillus nidulans

Gene. 2015 Dec 1;573(2):310-20. doi: 10.1016/j.gene.2015.07.066. Epub 2015 Jul 23.

Abstract

The kaeA(KAE1) (suDpro) gene, which was identified in Aspergillus nidulans as a suppressor of proline auxotrophic mutations, encodes the orthologue of Saccharomyces cerevisiae Kae1p, a member of the evolutionarily conserved KEOPS/EKC (Kinase, Endopeptidase and Other Proteins of Small size/Endopeptidase-like and Kinase associated to transcribed Chromatin) complex. In yeast, this complex has been shown to be involved in tRNA modification, transcription, and genome maintenance. In A. nidulans, mutations in kaeA result in several phenotypic effects, the derepression of arginine catabolism genes, and changes in the expression levels of several others, including genes involved in amino acid and siderophore metabolism, sulfate transport, carbon/energy metabolism, translation, and transcription regulation, such as rcoA(TUP1), which encodes the global transcriptional corepressor.

Keywords: Arginase; Kae1; Ornithine aminotransferase; rcoA(TUP1).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arginine / metabolism*
  • Aspergillus nidulans / genetics*
  • Aspergillus nidulans / metabolism
  • Base Sequence
  • Fungal Proteins / physiology*
  • Gene Expression Regulation, Fungal
  • Genes, Fungal
  • Genetic Pleiotropy
  • Metabolic Networks and Pathways
  • Molecular Sequence Data
  • Multiprotein Complexes / physiology
  • Mutation

Substances

  • Fungal Proteins
  • Multiprotein Complexes
  • Arginine