DNA-mediated supercharged fluorescent protein/graphene oxide interaction for label-free fluorescence assay of base excision repair enzyme activity

Chem Commun (Camb). 2015 Sep 7;51(69):13373-6. doi: 10.1039/c5cc04759e. Epub 2015 Jul 24.

Abstract

The interaction between supercharged green fluorescent protein (ScGFP) and graphene oxide (GO) as well as the resulting quenching effect of GO on ScGFP were investigated. Based on this unique quenching effect and the DNA-mediated ScGFP/GO interaction, a label-free fluorescence method has been established for homogeneously assaying the activity and inhibition of base excision repair enzyme.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / chemistry
  • DNA / metabolism*
  • DNA Repair
  • DNA Repair Enzymes / metabolism*
  • Fluorescent Dyes / chemistry
  • Graphite / chemistry*
  • Green Fluorescent Proteins / chemistry*
  • Green Fluorescent Proteins / metabolism
  • Oxides / chemistry
  • Spectrometry, Fluorescence
  • Uracil-DNA Glycosidase / metabolism

Substances

  • Fluorescent Dyes
  • Oxides
  • Green Fluorescent Proteins
  • Graphite
  • DNA
  • Uracil-DNA Glycosidase
  • DNA Repair Enzymes