New fluorescence probes for biomolecules

Katarzyna Jurek; Janina Kabatc; Katarzyna Kostrzewska; Marlena Grabowska

doi:10.3390/molecules200713071

New fluorescence probes for biomolecules

Molecules. 2015 Jul 20;20(7):13071-9. doi: 10.3390/molecules200713071.

Authors

Katarzyna Jurek¹, Janina Kabatc², Katarzyna Kostrzewska³, Marlena Grabowska⁴

Affiliations

¹ Faculty of Chemical Technology and Engineering, University of Science and Technology, UTP, Bydgoszcz 85-326, Poland. katarzyna.jurek@utp.edu.pl.
² Faculty of Chemical Technology and Engineering, University of Science and Technology, UTP, Bydgoszcz 85-326, Poland. nina@utp.edu.pl.
³ Faculty of Chemical Technology and Engineering, University of Science and Technology, UTP, Bydgoszcz 85-326, Poland. katarzyna.kostrzewska@utp.edu.pl.
⁴ Faculty of Chemical Technology and Engineering, University of Science and Technology, UTP, Bydgoszcz 85-326, Poland. marlena.grabowska@o2.pl.

Abstract

Steady state fluorescence measurements have been used for the investigation of interaction between the bovine serum albumin (BSA) and fluorescence probes: 3-hydroxy-2,4- bis[(3-methyl-1,3-benzoxazol-2(3H)-ylidene)methyl]cyclobut-2-en-1-one (SQ6), 3-hydroxy- 2,4-bis[(3-methyl-1,3-benzothiazol-2(3H)-ylidene)methyl]cyclobut-2-en-1-one (SQ7) and 3-hydroxy-2,4-bis[(1,3,3-trimethyl-1,3-dihydro-2H-indol-2-ylidene)methyl]cyclobut-2-en-1-one (SQ8). The binding constant between bovine serum albumin and squarine dyes has been determined by using both the Benesi-Hildebrand and Stern-Volmer equations. The negative value of free energy change indicates the existence of a spontaneous complexation process of BSA with squarine dyes.

Keywords: biomolecule determination; bovine serum albumin; fluorescence method; fluorescence probe; squarine dyes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Fluorescent Dyes / chemical synthesis*
Fluorescent Dyes / chemistry*
Serum Albumin, Bovine / chemistry*

Substances

Fluorescent Dyes
Serum Albumin, Bovine