Soybean isoflavone treatment induces osteoblast differentiation and proliferation by regulating analysis of Wnt/β-catenin pathway

Gene. 2015 Dec 1;573(2):273-7. doi: 10.1016/j.gene.2015.07.054. Epub 2015 Jul 17.

Abstract

It has been reported that soybean isoflavones (SI) have anti-bone resorptive activity in vivo. However, little is known about the cellular and molecular mechanisms of this effect. In this study, we investigated the effects of SI on osteoblast differentiation and proliferation. Results showed that SI promoted osteoblast proliferation and differentiation which could be marked as the formation of hydroxyapatite crystals or extracellular matrix and increased ALP level. SI treatment markedly decreased RANKL levels and increased OPG levels in osteoblast cells. SI resulted in activation of the Wnt/β-catenin pathway. These results demonstrate that SI may be a useful remedy for the treatment of osteoporosis.

Keywords: RANKL; Soybean isoflavones; Wnt/β-catenin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / metabolism
  • Animals
  • Cell Differentiation / drug effects
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Glycine max / chemistry
  • Isoflavones / pharmacology*
  • Osteoblasts / drug effects
  • Osteoblasts / physiology*
  • Osteoprotegerin / metabolism
  • Plant Extracts / pharmacology*
  • RANK Ligand / metabolism
  • Rats
  • Wnt Proteins / genetics
  • Wnt Proteins / metabolism
  • Wnt Signaling Pathway*
  • Wnt3A Protein / genetics
  • Wnt3A Protein / metabolism
  • beta Catenin / genetics
  • beta Catenin / metabolism

Substances

  • Ctnnb1 protein, rat
  • Isoflavones
  • Osteoprotegerin
  • Plant Extracts
  • RANK Ligand
  • Tnfrsf11b protein, rat
  • Wnt Proteins
  • Wnt3A Protein
  • Wnt7b protein, rat
  • beta Catenin
  • Alkaline Phosphatase