Rapid and sensitive method for analysis of nitrate in meat samples using ultra performance liquid chromatography-mass spectrometry

Masoom Raza Siddiqui; Saikh Mohammad Wabaidur; Zeid A ALOthman; M Z A Rafiquee

doi:10.1016/j.saa.2015.07.028

Rapid and sensitive method for analysis of nitrate in meat samples using ultra performance liquid chromatography-mass spectrometry

Spectrochim Acta A Mol Biomol Spectrosc. 2015 Dec 5:151:861-6. doi: 10.1016/j.saa.2015.07.028. Epub 2015 Jul 7.

Authors

Masoom Raza Siddiqui¹, Saikh Mohammad Wabaidur², Zeid A ALOthman², M Z A Rafiquee³

Affiliations

¹ Advanced Materials Research Chair, Chemistry Department, College of Science, King Saud University, P.O. Box 2455, 11451 Riyadh, Saudi Arabia. Electronic address: siddiqui124@gmail.com.
² Advanced Materials Research Chair, Chemistry Department, College of Science, King Saud University, P.O. Box 2455, 11451 Riyadh, Saudi Arabia.
³ Chemistry Department, College of Science, King Saud University, P.O. Box 2455, 11451 Riyadh, Saudi Arabia; Department of Applied Chemistry, Aligarh Muslim University, Aligarh 202002, India.

PMID: 26184470
DOI: 10.1016/j.saa.2015.07.028

Abstract

A sensitive and selective ultra performance liquid chromatography-mass spectrometric method has been developed for the quantitative analysis of nitrate in meat samples. Selected ion reaction (SIR) mode was adopted to identify and quantify the nitrate. Chromatographic analyses were performed on a BEH C-18 column with a mobile phase consisting of a surfactant (Cetylpyridinium chloride) and acetonitrile in equal ratio (50/50, v/v) at a flow rate of 0.4 mL min(-1). The limit of detection and limit of quantitation of the developed method was found to be 0.0599 and 0.1817 mg kg(-1), respectively. The linearity of the proposed method was checked in the concentration range of 0.5-10 mg kg(-1) with an excellent correlation coefficient (r) of 0.997. The recovery of the nitrate in the meat samples were in the range of 98.02-98.99%.

Keywords: Mass spectrometry; Meat; Nitrate; Ultra performance liquid chromatography.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Calibration
Cetylpyridinium / chemistry
Chromatography, High Pressure Liquid / methods*
Food Analysis / methods*
Limit of Detection
Meat / analysis*
Nitrates / analysis*
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization / methods*

Substances

Nitrates
Cetylpyridinium