Abstract
Epidermal growth factor (EGF) receptor capping results from the interaction between the receptors and polyvalent ligands in A-431 cells examined in suspension at 22 degrees C. Colocalization of actin and spectrin with the ligand-receptor complexes during the redistribution was shown using double immunofluorescence. The obtained data show that the cortical microfilaments are involved in capping. EGF receptors become associated with the Triton-insoluble cytoskeleton as a consequence of ligand binding. EGF-receptor capping is not sensitive to the action of cytochalasin B. Capping in A-431 cells is discussed as a new model for studying the redistribution of the ligand-receptor complex.
MeSH terms
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Actins / physiology
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Antibodies / pharmacology
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Antibodies, Monoclonal / pharmacology
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Carcinoma, Squamous Cell / physiopathology*
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Cell Line
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Cytochalasin B / pharmacology
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Cytoskeleton / drug effects
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Cytoskeleton / physiology*
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Epidermal Growth Factor / immunology
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Epidermal Growth Factor / pharmacology
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ErbB Receptors / drug effects
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ErbB Receptors / physiology*
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Humans
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Immune Sera / pharmacology
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Ligands
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Receptor Aggregation / drug effects
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Receptor Aggregation / physiology*
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Spectrin / physiology
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Tumor Cells, Cultured / drug effects
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Tumor Cells, Cultured / physiology
Substances
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Actins
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Antibodies
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Antibodies, Monoclonal
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Immune Sera
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Ligands
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Spectrin
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Cytochalasin B
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Epidermal Growth Factor
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ErbB Receptors