[Detection of the BCR-ABL fusion: Comparative analysis of six commercially available FISH fusion probes]

Pathologe. 2015 Jul;36(4):372-84. doi: 10.1007/s00292-015-0040-0.
[Article in German]

Abstract

Chronic myeloid leukemia (CML) is diagnostically defined by the reciprocal translocation t(9;22)(q34;q11). This aberration can be detected by the BCR-ABL fluorescence in situ hybridization (FISH) technique. This article presents a comparative analysis of different commercially available FISH probes and different FISH protocols in order to optimize this technique on formalin-fixed and paraffin-embedded bone marrow trephine biopsies.

Publication types

  • Comparative Study
  • English Abstract

MeSH terms

  • Biopsy, Needle
  • Bone Marrow / pathology*
  • Chromosomes, Human, Pair 22
  • Chromosomes, Human, Pair 9
  • Fusion Proteins, bcr-abl / analysis*
  • Fusion Proteins, bcr-abl / genetics*
  • Humans
  • In Situ Hybridization, Fluorescence / instrumentation
  • In Situ Hybridization, Fluorescence / methods*
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / pathology*
  • Sensitivity and Specificity
  • Translocation, Genetic

Substances

  • Fusion Proteins, bcr-abl