Genetic and morphological characterization of Trichuris myocastoris found in Myocastor coypus in the Czech Republic

Parasitol Res. 2015 Nov;114(11):3969-75. doi: 10.1007/s00436-015-4623-8. Epub 2015 Jul 15.

Abstract

Trichuris sp. individuals were collected from Myocastor coypus from fancy breeder farms in the Czech Republic. Using morphological and biometrical methods, 30 female and 30 male nematodes were identified as Trichuris myocastoris. This paper presents the first molecular description of this species. The ribosomal DNA (rDNA) region, consisting of internal transcribed spacer (ITS)-1, 5.8 gene and ITS-2, was sequenced. Based on an analysis of 651 bp, T. myocastoris was found to be different from any other Trichuris species for which published sequencing of the ITS region is available. The phylogenetic relationships were estimated using the maximum parsimony methods and Bayesian analyses. T. myocastoris was found to be significantly closely related to Trichuris of rodents than those of ruminants.

Keywords: ITS1-5.8S-ITS2; Myocastor coypus; Parasites; Trichuris myocastoris; rDNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Bayes Theorem
  • Czech Republic / epidemiology
  • DNA, Helminth / chemistry
  • DNA, Helminth / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • DNA, Ribosomal Spacer / chemistry
  • DNA, Ribosomal Spacer / genetics
  • Female
  • Humans
  • Male
  • Phylogeny
  • Trichuriasis / epidemiology
  • Trichuriasis / veterinary*
  • Trichuriasis / virology
  • Trichuris* / anatomy & histology
  • Trichuris* / classification
  • Trichuris* / genetics
  • Trichuris* / isolation & purification

Substances

  • DNA, Helminth
  • DNA, Ribosomal
  • DNA, Ribosomal Spacer