Biological evaluation of secondary metabolites from the root of Machilus obovatifolia

Chem Biodivers. 2015 Jul;12(7):1057-67. doi: 10.1002/cbdv.201400196.

Abstract

Bioassay-guided fractionation of the root of Machilus obovatifolia led to the isolation of four new lignans, epihenricine B (1), threo-(7'R,8'R) and threo-(7'S,8'S)-methylmachilusol D (2 and 3), and isofragransol A (4), along with 23 known compounds. The compounds were obtained as isomeric mixtures (i.e., 2/3 and 4/20, resp.). The structures were elucidated by spectral analyses. Among the isolates, 1, licarin A (12), guaiacin (14), (±)-syringaresinol (21), and (-)-epicatechin (23) showed ABTS (=2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical-scavenging activity, with SC50 values of 11.7±0.5, 12.3±1.1, 11.0±0.1, 10.6±0.3, and 9.5±0.2 μM in 20 min, respectively. In addition, kachirachirol B (17) showed cytotoxicity against the NCI-H460 cell line with an IC50 value of 3.1 μg/ml.

Keywords: Antioxidant activity; Cytotoxic activity; Machilus obovatifolia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Dose-Response Relationship, Drug
  • Hep G2 Cells
  • Humans
  • Lauraceae / chemistry*
  • Lauraceae / metabolism
  • Lignans / chemistry
  • Lignans / isolation & purification
  • Lignans / metabolism*
  • MCF-7 Cells
  • Molecular Conformation
  • Plant Roots / chemistry*
  • Plant Roots / metabolism
  • Structure-Activity Relationship

Substances

  • Lignans