Analysis of liver fragment subjected to autologous transplant at rat's retroperitoneum

Abstract

Background: To investigate the regeneration process of autologous implants of liver on the retroperitoneum.

Methods: Thirty male Fisher rats were used divided in to group 1 (G1): studied 60 d after surgery; group 2 (G2): studied 90 d after surgery; group 3 (G3): studied 180 d after surgery; and group C (GC): animals without surgery. Hepatic fragment was processed for histologic and biochemical analysis.

Results: There was inflammatory infiltrate, diffuse hydropic degeneration, necrosis, and moderate fibrosis that reduced in direct relation to the postsurgical time. The concentration of albumin was different between GC and G1 and between G1 and G3 (P = 0.0007). The Catalase (CAT) was related to the time of surgery with GC being different when compared with G1, G2, and G3 (P < 0.0001). The oxidative stress measured through the thiobarbituric acid reactive substances lipid peroxidation was different between the GC and the G2 groups (P = 0.0381).

Conclusions: The analysis made showed hepatic regeneration in the fragment subjected to autologous transplant at the retroperitoneum.

Keywords: Autotransplantation; Experimental surgery; Hepatic regeneration.