In Situ Proximity Ligation Assay (PLA)

Methods Mol Biol. 2015:1318:149-59. doi: 10.1007/978-1-4939-2742-5_15.

Abstract

In situ proximity ligation assay (PLA) is a method to identify physical closeness of proteins, where a signal will only be produced if the two proteins are closer than 40 nm, in tissue section or cell cultures. Modifications of the PLA method can also be used to increase specificity or sensitivity of standard immunohistochemistry protocols.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / chemistry*
  • Antibodies / isolation & purification
  • Biological Assay / instrumentation
  • Biological Assay / methods*
  • Brain Chemistry
  • Fluorescence
  • Immunohistochemistry / instrumentation
  • Immunohistochemistry / methods*
  • Mice
  • Molecular Imaging
  • Nerve Tissue Proteins / analysis*
  • Nerve Tissue Proteins / chemistry
  • Oligonucleotides / chemical synthesis
  • Oligonucleotides / chemistry*
  • Protein Interaction Mapping
  • Rabbits
  • Sensitivity and Specificity

Substances

  • Antibodies
  • Nerve Tissue Proteins
  • Oligonucleotides