Collision cross sections of high-mannose N-glycans in commonly observed adduct states--identification of gas-phase conformers unique to [M-H](-) ions

Analyst. 2015 Oct 21;140(20):6799-803. doi: 10.1039/c5an01092f.

Abstract

We report collision cross sections (CCS) of high-mannose N-glycans as [M + Na](+), [M + K](+), [M + H](+), [M + Cl](-), [M + H2PO4](-) and [M - H](-) ions, measured by drift tube (DT) ion mobility-mass spectrometry (IM-MS) in helium and nitrogen gases. Further analysis using traveling wave (TW) IM-MS reveal the existence of distinct conformers exclusive to [M - H](-) ions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carbohydrate Conformation
  • Cattle
  • Helium / chemistry*
  • Mannose / chemistry*
  • Mass Spectrometry*
  • Nitrogen / chemistry*
  • Polysaccharides / chemistry*

Substances

  • Polysaccharides
  • Helium
  • Nitrogen
  • Mannose