Biotunable acoustic node assembly of organoids

Pu Chen; Sinan Güven; Osman Berk Usta; Martin L Yarmush; Utkan Demirci

doi:10.1002/adhm.201500279

Biotunable acoustic node assembly of organoids

Adv Healthc Mater. 2015 Sep 16;4(13):1937-43. doi: 10.1002/adhm.201500279. Epub 2015 Jul 7.

Authors

Pu Chen¹, Sinan Güven¹, Osman Berk Usta², Martin L Yarmush^{2

3}, Utkan Demirci¹

Affiliations

¹ Bio-Acoustic MEMS in Medicine (BAMM) Lab, Canary Center at Stanford for Early Cancer Detection, Department of Radiology, School of Medicine, Stanford University, Palo Alto, CA, 94304, USA.
² Center for Engineering in Medicine at Massachusetts General Hospital, Harvard Medical School and Shriners Hospital for Children, 51 Blossom St., Boston, MA, 02114, USA.
³ Department of Biomedical Engineering, Rutgers University, 599 Taylor Rd., Piscataway, NJ, 08854, USA.

Abstract

Bioengineering of 3D microtissues from cell spheroids is demonstrated by employing the vibration of acoustic standing waves and its hydrodynamic effect at the bottom of a liquid-carrier chamber. A large number of cell spheroids (>10(4) ) are assembled in seconds into a closely packed structure in a scaffold-free fashion under nodal pattern of the standing waves in a fluidic environment.

Keywords: 3D co-culture; cell spheroids; scaffold-free assembly; spheroid fusion; tissue engineering.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Acoustics
Animals
Cell Survival
Cells, Cultured
Hepatocytes / cytology
Hepatocytes / metabolism
Human Umbilical Vein Endothelial Cells
Humans
Organoids / cytology*
Organoids / metabolism
Rats
Spheroids, Cellular / cytology
Tissue Engineering

Abstract

Publication types

MeSH terms

Grants and funding