Hybrid core/shell microparticles and their use for understanding biological processes

Akash Bachhuka; Susan N Christo; Alex Cavallaro; Kerrilyn R Diener; Agnieszka Mierczynska; Louise E Smith; Romeo Marian; Jim Manavis; John D Hayball; Krasimir Vasilev

doi:10.1016/j.jcis.2015.06.040

Hybrid core/shell microparticles and their use for understanding biological processes

J Colloid Interface Sci. 2015 Nov 1:457:9-17. doi: 10.1016/j.jcis.2015.06.040. Epub 2015 Jun 29.

Authors

Affiliations

¹ Mawson Institute, University of South Australia, Adelaide, SA 5000, Australia.
² Experimental Therapeutics Laboratory, Royal Adelaide Hospital, Adelaide, SA 5000, Australia; Sansom Institute, University of South Australia, Adelaide, SA 5000, Australia.
³ Mawson Institute, University of South Australia, Adelaide, SA 5000, Australia; School of Engineering, University of South Australia, Adelaide, SA 5000, Australia.
⁴ Experimental Therapeutics Laboratory, Royal Adelaide Hospital, Adelaide, SA 5000, Australia; Robinson Research Institute, School of Paediatrics and Reproductive Health, University of Adelaide, Adelaide, SA 5005, Australia.
⁵ The Australian Wine Research Institute, Urrbrae, Adelaide, SA 5000, Australia.
⁶ School of Engineering, University of South Australia, Adelaide, SA 5000, Australia.
⁷ Centre for Neurological Diseases, SA Pathology, Adelaide, SA 5000, Australia.
⁸ Experimental Therapeutics Laboratory, Royal Adelaide Hospital, Adelaide, SA 5000, Australia; Sansom Institute, University of South Australia, Adelaide, SA 5000, Australia; School of Medicine, University of Adelaide, Adelaide, SA 5005, Australia. Electronic address: john.hayball@unisa.edu.au.
⁹ Mawson Institute, University of South Australia, Adelaide, SA 5000, Australia; School of Engineering, University of South Australia, Adelaide, SA 5000, Australia. Electronic address: krasimir.vasilev@unisa.edu.au.

PMID: 26149416
DOI: 10.1016/j.jcis.2015.06.040

Abstract

Hybrid micro and nanoparticles have become a topic of intense research in recent years. This is due to the special properties of these materials that open new avenues in advanced applications. Herein, we report a novel method for the generation of hybrid particles utilising plasma polymerization. Poly (methyl methacrylate) (PMMA) beads were first coated with a thin allylamine based plasma polymer layer. Gold nanoparticles of engineered size and surface structure were then attached in a controlled manner to the plasma polymer coated beads. To generate uniform chemistry on the outermost surface and to preserve the nanotopography, we deposited a 5-10 nm thin layer of Acpp. We demonstrated that these particles can be utilized in in vivo models to interrogate important biological phenomena. Specifically, we used them in mice to study the inflammatory and foreign body responses to surface nanotopography. The data strongly indicates that surface nanotopography and chemistry can modulate collagen production and the number of adhering immune cells. The method for generating hybrid particles reported here is solvent free and can open new opportunities in fields such as tissue engineering, drug delivery, biosensors, and regenerative medicine.

Keywords: Hybrid particles; Macrophages; Nanoparticles; Nanotopography; Neutrophils; Plasma polymerization; Surface chemistry.

MeSH terms

Allylamine / chemistry*
Animals
Foreign-Body Reaction
Gold / chemistry*
Inflammation / metabolism*
Metal Nanoparticles / chemistry*
Mice
Mice, Inbred C57BL
Microchemistry*
Particle Size
Polymethyl Methacrylate / chemistry*
Surface Properties

Substances

Allylamine
Gold
Polymethyl Methacrylate