MoS2 nanosheet-based fluorescent biosensor for protein detection via terminal protection of small-molecule-linked DNA and exonuclease III-aided DNA recycling amplification

Biosens Bioelectron. 2015 Dec 15:74:227-32. doi: 10.1016/j.bios.2015.06.045. Epub 2015 Jun 23.

Abstract

A new MoS2 nanosheet-based fluorescent biosensor for protein detection is developed. This method combines the terminal protection of small-molecule-linked DNA (TPSMLD) and exonuclease III (Exo III)-aided DNA recycling amplification to convert protein assay into the highly sensitive detection of DNA. Taking the streptavidin (SA)-biotin system as a model, a detection limit of 0.67 ng mL(-1) SA is obtained with a good selectivity. The study demonstrated here not only offers simple, sensitive and selective detection method for protein assay, but also will expand the application of the emerging 2D nanomaterials into biological assay.

Keywords: Exonuclease III; Fluorescent biosensor; MoS(2) nanosheet; Protein detection; Terminal protection of small-molecule-linked DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / methods*
  • Biotin / chemistry
  • DNA / analysis*
  • Disulfides / chemistry*
  • Exodeoxyribonucleases / chemistry*
  • Humans
  • Limit of Detection
  • Molybdenum / chemistry*
  • Nanostructures / chemistry*
  • Nucleic Acid Amplification Techniques / methods
  • Spectrometry, Fluorescence / methods
  • Streptavidin / analysis*
  • Streptavidin / blood

Substances

  • Disulfides
  • Biotin
  • Molybdenum
  • DNA
  • Streptavidin
  • Exodeoxyribonucleases
  • exodeoxyribonuclease III
  • molybdenum disulfide