Abstract
The generation of patient-specific induced pluripotent stem (iPS) cells permits the development of next-generation patient-specific systems biology models reflecting personalized genomics profiles to better understand pathophysiology. In this chapter, we describe how to create a patient-specific iPS cell line. There are three major steps: (1) performing a skin biopsy procedure on the patient; (2) extracting human fibroblast cells from the skin biopsy tissue; and (3) reprogramming patient-specific fibroblast cells into the pluripotent stem cell stage.
Keywords:
Fibroblast; Human iPS; Reprogramming; Sendai virus; Skin biopsy.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Biological Assay / instrumentation
-
Biological Assay / methods*
-
Biopsy
-
Cell Differentiation
-
Cell Line
-
Cellular Reprogramming*
-
Fibroblasts / metabolism
-
Fibroblasts / pathology*
-
Gene Expression
-
Genetic Vectors / chemistry
-
Genetic Vectors / metabolism
-
Humans
-
Induced Pluripotent Stem Cells / metabolism*
-
Induced Pluripotent Stem Cells / pathology
-
Kruppel-Like Factor 4
-
Kruppel-Like Transcription Factors / genetics
-
Kruppel-Like Transcription Factors / metabolism
-
Octamer Transcription Factor-3 / genetics
-
Octamer Transcription Factor-3 / metabolism
-
Primary Cell Culture
-
Proto-Oncogene Proteins c-myc / genetics
-
Proto-Oncogene Proteins c-myc / metabolism
-
SOXB1 Transcription Factors / genetics
-
SOXB1 Transcription Factors / metabolism
-
Sendai virus / genetics
-
Skin / metabolism
-
Skin / pathology
-
Teratoma / genetics
-
Teratoma / metabolism
-
Teratoma / pathology
-
Transgenes*
Substances
-
Kruppel-Like Factor 4
-
Kruppel-Like Transcription Factors
-
MYC protein, human
-
Octamer Transcription Factor-3
-
POU5F1 protein, human
-
Proto-Oncogene Proteins c-myc
-
SOX2 protein, human
-
SOXB1 Transcription Factors