Abstract
Development of immunoblots is commonly performed using enzyme-labeled antibodies which convert soluble substrates into insoluble colored products. A simple, rapid, and sensitive alternative method which produces low background and allows a rapid quantitative evaluation is the use of radiolabeled antibodies or protein A conjugates. Here we describe the use of iodinated secondary antibodies for immunodetection of an autoantigen during HPLC purification.
MeSH terms
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Autoantigens / analysis*
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Autoantigens / isolation & purification
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Blotting, Western / methods
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Chromatography, High Pressure Liquid / methods
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Electrophoresis, Polyacrylamide Gel / methods
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Halogenation
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HeLa Cells
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Humans
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Immunoblotting / methods*
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Immunoconjugates / chemistry*
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Indicators and Reagents / chemistry
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Iodine Radioisotopes / chemistry*
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Ribonucleoproteins / analysis*
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Ribonucleoproteins / isolation & purification
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SS-B Antigen
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Staphylococcal Protein A / chemistry
Substances
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Autoantigens
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Immunoconjugates
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Indicators and Reagents
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Iodine Radioisotopes
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Ribonucleoproteins
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Staphylococcal Protein A