CLIP-ASAP-HSQC for fast and accurate extraction of one-bond couplings from isotropic and partially aligned molecules

Johanna Becker; Burkhard Luy

doi:10.1002/mrc.4276

CLIP-ASAP-HSQC for fast and accurate extraction of one-bond couplings from isotropic and partially aligned molecules

Magn Reson Chem. 2015 Nov;53(11):878-85. doi: 10.1002/mrc.4276. Epub 2015 Jul 1.

Authors

Johanna Becker¹, Burkhard Luy^{1

2}

Affiliations

¹ Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces 4 - Magnetic Resonance, Eggenstein-Leopoldshafen, Germany.
² Karlsruhe Institute of Technology (KIT), Institute of Organic Chemistry, Karlsruhe, Germany.

PMID: 26137959
DOI: 10.1002/mrc.4276

Abstract

Fast measurement of heteronuclear one-bond couplings, a class of NMR parameters valuable for structure elucidation, is highly desirable, especially if samples undergo chemical reactions or dynamic processes are observed. Methods presented so far face severe limitations in terms of resolution, accessible bandwidth, and sensitivity. We present the CLean InPhase-Acceleration by Sharing Adjacent Polarization-HSQC (CLIP-ASAP-HSQC) pulse sequence that allows fast acquisition of spectra with clean inphase multiplets in about 25 s. The performance in terms of accurate extraction of one-bond couplings is demonstrated on three test samples including partially aligned molecules.

Keywords: ASAP approach; HSQC; RDCs; coupling constants; fast experiments; partial alignment; pure absorption; shaped pulses.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbon Isotopes / chemistry
Chemistry Techniques, Analytical / methods*
Nuclear Magnetic Resonance, Biomolecular*
Time Factors

Substances

Carbon Isotopes