Nanozyme-strip for rapid local diagnosis of Ebola

Demin Duan; Kelong Fan; Dexi Zhang; Shuguang Tan; Mifang Liang; Yang Liu; Jianlin Zhang; Panhe Zhang; Wei Liu; Xiangguo Qiu; Gary P Kobinger; George Fu Gao; Xiyun Yan

doi:10.1016/j.bios.2015.05.025

Nanozyme-strip for rapid local diagnosis of Ebola

Biosens Bioelectron. 2015 Dec 15:74:134-41. doi: 10.1016/j.bios.2015.05.025. Epub 2015 May 11.

Authors

Demin Duan¹, Kelong Fan¹, Dexi Zhang¹, Shuguang Tan², Mifang Liang³, Yang Liu³, Jianlin Zhang¹, Panhe Zhang⁴, Wei Liu⁴, Xiangguo Qiu⁵, Gary P Kobinger⁵, George Fu Gao⁶, Xiyun Yan⁷

Affiliations

¹ Key Laboratory of Protein and Peptide Pharmaceuticals, CAS-University of Tokyo Joint Laboratory of Structural Virology and Immunology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
² CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
³ National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
⁴ State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
⁵ National Laboratory for Zoonotic Diseases and Special Pathogens, Public Health Agency of Canada, Winnipeg, Manitoba R3E 3R2, Canada.
⁶ CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Office of Director-General, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
⁷ Key Laboratory of Protein and Peptide Pharmaceuticals, CAS-University of Tokyo Joint Laboratory of Structural Virology and Immunology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: yanxy@ibp.ac.cn.

PMID: 26134291
DOI: 10.1016/j.bios.2015.05.025

Abstract

Ebola continues to rage in West Africa. In the absence of an approved vaccine or treatment, the priority in controlling this epidemic is to promptly identify and isolate infected individuals. To this end, a rapid, highly sensitive, and easy-to-use test for Ebola diagnosis is urgently needed. Here, by using Fe3O4 magnetic nanoparticle (MNP) as a nanozyme probe, we developed a MNP-based immunochromatographic strip (Nanozyme-strip), which detects the glycoprotein of Ebola virus (EBOV) as low as 1 ng/mL, which is 100-fold more sensitive than the standard strip method. The sensitivity of the Nanozyme-strip for EBOV detection and diagnostic accuracy for New Bunyavirus clinical samples is comparable with ELISA, but is much faster (within 30 min) and simpler (without need of specialist facilities). The results demonstrate that the Nanozyme-strip test can rapidly and sensitively detect EBOV, providing a valuable simple screening tool for diagnosis of infection in Ebola-stricken areas.

Keywords: Ebola detection; Immunochromatographic strip; Nanozyme.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Affinity / instrumentation*
Ebolavirus / isolation & purification*
Equipment Design
Glycoproteins / isolation & purification*
Hemorrhagic Fever, Ebola / diagnosis*
Hemorrhagic Fever, Ebola / virology
Humans
Magnetite Nanoparticles / chemistry*
Reagent Strips / analysis*
Sensitivity and Specificity
Viral Proteins / isolation & purification*

Substances

Glycoproteins
Magnetite Nanoparticles
Reagent Strips
Viral Proteins