Three new species and one new subspecies of earthworms from Hainan Island, China, are described: Amynthas dinganensis sp. nov., Amynthas tenuis sp. nov., Metaphire wuzhimontis sp. nov. and Metaphire magna minuscula subsp. nov. Amynthas dinganensis and Amynthas tenuis have two pairs of spermathecal pores in 5/6-6/7, and Metaphire wuzhimontis and Metaphire magna minuscula have two pairs of spermathecal pores in 7/8-8/9. COI and 16S gene fragments of the new species taxa have been sequenced. We provide comparisons between these new species taxa and related species using morphological and molecular characters.