A new species of Enderleina (Plecoptera), E. khazeni n. sp., is described from two males collected in Bolivar Province, Southeast Venezuela. It appears to be more closely related to E. preclara Jewett that to other Enderleina species, but can be clearly distinguished from that species by shape of the penial sclerites. With the description of this new taxon, six species of the genus are known, of these males of four of them have been described.