Comparison of in vitro methods for the production of Paenibacillus larvae endospores

Israel Alvarado; Michelle M Elekonich; Ernesto Abel-Santos; Helen J Wing

doi:10.1016/j.mimet.2015.06.011

Comparison of in vitro methods for the production of Paenibacillus larvae endospores

J Microbiol Methods. 2015 Sep:116:30-2. doi: 10.1016/j.mimet.2015.06.011. Epub 2015 Jun 27.

Authors

Israel Alvarado¹, Michelle M Elekonich², Ernesto Abel-Santos³, Helen J Wing⁴

Affiliations

¹ School of Life Sciences, 4505 South Maryland Parkway, Bldg WHI, M/S 4004, University of Nevada, Las Vegas, Las Vegas, NV 89154-4004, United States. Electronic address: alvara95@unlv.nevada.edu.
² School of Life Sciences, 4505 South Maryland Parkway, Bldg WHI, M/S 4004, University of Nevada, Las Vegas, Las Vegas, NV 89154-4004, United States. Electronic address: melekoni@nsf.gov.
³ Department of Chemistry, 4505 South Maryland Parkway, Bldg CHE, M/S 4003, University of Nevada, Las Vegas, Las Vegas, NV 89154-4003, United States. Electronic address: ernesto.abelsantos@unlv.edu.
⁴ School of Life Sciences, 4505 South Maryland Parkway, Bldg WHI, M/S 4004, University of Nevada, Las Vegas, Las Vegas, NV 89154-4004, United States. Electronic address: helen.wing@unlv.edu.

PMID: 26130193
DOI: 10.1016/j.mimet.2015.06.011

Abstract

Paenibacillus larvae endospores are the infectious particles of the honey bee brood disease, American Foulbrood. We demonstrate that our previously published protocol (Alvarado et al., 2013) consistently yields higher numbers and purer preparations of P. larvae endospores, than previously described protocols, regardless of the strain tested (B-3650, B-3554 or B-3685).

Keywords: American Foulbrood disease; Honey bee; Paenibacillus larvae; Spore production.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bees / microbiology*
Paenibacillus / physiology*
Spores, Bacterial / growth & development*
Spores, Bacterial / ultrastructure