Rotation is the primary motion of paired human epidermal keratinocytes

Sota Tate; Matome Imai; Natsuki Matsushita; Emi K Nishimura; Shigeki Higashiyama; Daisuke Nanba

doi:10.1016/j.jdermsci.2015.05.008

Rotation is the primary motion of paired human epidermal keratinocytes

J Dermatol Sci. 2015 Sep;79(3):194-202. doi: 10.1016/j.jdermsci.2015.05.008. Epub 2015 Jun 10.

Authors

Sota Tate¹, Matome Imai¹, Natsuki Matsushita², Emi K Nishimura³, Shigeki Higashiyama⁴, Daisuke Nanba⁵

Affiliations

¹ Department of Biochemistry and Molecular Genetics, Graduate School of Medicine, Ehime University, Shitsukawa, Toon, Ehime 791-0295, Japan.
² Translational Research Center, Ehime University Hospital, Ehime University, Shitsukawa, Toon, Ehime 791-0295, Japan.
³ Department of Stem Cell Biology, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
⁴ Department of Biochemistry and Molecular Genetics, Graduate School of Medicine, Ehime University, Shitsukawa, Toon, Ehime 791-0295, Japan; Division of Cell Growth and Tumor Regulation, Proteo-Science Center (PROS), Ehime University, Shitsukawa, Toon, Ehime 791-0295, Japan.
⁵ Department of Biochemistry and Molecular Genetics, Graduate School of Medicine, Ehime University, Shitsukawa, Toon, Ehime 791-0295, Japan; Division of Cell Growth and Tumor Regulation, Proteo-Science Center (PROS), Ehime University, Shitsukawa, Toon, Ehime 791-0295, Japan; Department of Stem Cell Biology, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan. Electronic address: nanbscm@tmd.ac.jp.

PMID: 26126707
DOI: 10.1016/j.jdermsci.2015.05.008

Abstract

Background: Collective motion of keratinocytes is involved in morphogenesis, homeostasis, and wound healing of the epidermis. Yet how the collective motion of keratinocytes emerges from the behavior of individual cells is still largely unknown.

Objective: The aim of this study was to find the cellular behavior that links single and collective motion of keratinocytes.

Methods: We investigated the behavior of two-cell colonies of HaCaT keratinocytes by a combination of time-lapse imaging and image processing.

Results: The two-cell colonies of HaCaT cells were formed as a contacted pair of keratinocyte clones. Image analysis and cell culture experiments revealed that the rotational speed of two-cell colonies was positively associated with their proliferative capacity. α6 integrin was required for the rotational motion of two-cell keratinocyte colonies. We also confirmed that two-cell colonies of keratinocytes predominantly exhibited the rotational, but not translational, motion, two modes of motion in a contact pair of rotating objects.

Conclusion: The rotational motion is the primary motion of two-cell keratinocyte colonies and its speed is positively associated with their proliferative capacity. This study suggests that the assembly of rotating keratinocytes generates the collective motion of proliferative keratinocytes during morphogenesis and wound healing of the epidermis.

Keywords: Cellular dynamics; Hacat cells; Keratinocytes; Proliferative capacity; Rotational motion.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Cell Movement / genetics
Cell Movement / physiology*
Cell Proliferation
Clone Cells
Coculture Techniques
Humans
Image Processing, Computer-Assisted
Integrin alpha6 / genetics*
Keratinocytes / physiology*
Rotation
Time-Lapse Imaging

Substances

Integrin alpha6