Formulations for immobilized pH gradients including pH extremes

E Gianazza; F Celentano; S Magenes; C Ettori; P G Righetti

doi:10.1002/elps.1150101115

Formulations for immobilized pH gradients including pH extremes

Electrophoresis. 1989 Nov;10(11):806-8. doi: 10.1002/elps.1150101115.

Authors

E Gianazza¹, F Celentano, S Magenes, C Ettori, P G Righetti

Affiliation

¹ Faculty of Pharmacy, Department of Biomedical Sciences and Technologies, University of Milan, Italy.

PMID: 2612482
DOI: 10.1002/elps.1150101115

Abstract

Formulations are given both for narrow (less than 2 pH units) and for wide range (up to 8 pH units) immobilized pH gradients, spanning between pH 2.5 and pH 11. The contribution from water to the buffering power (beta) at these pH extremes requires the recipes to be optimized (in terms of gradient linearity) for each desired level of beta av.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Buffers
Electrophoresis / methods*
Hydrogen-Ion Concentration*

Substances

Buffers