Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning

Small. 2015 Sep;11(36):4643-50. doi: 10.1002/smll.201500674. Epub 2015 Jun 29.

Abstract

Living-cell-based screens can facilitate lead discovery of functional therapeutics of interest. A versatile and scalable method is reported that uses dense arrays of nanowells for imparting defined patterns on monolayers of cells. It is shown that this approach can coordinate a multi-component biological assay by designing and implementing a high-throughput, functional nanoliter-scale neutralization assay to identify neutralizing antibodies against HIV.

Keywords: cell patterning; human immunodeficiency virus (HIV); living-cell arrays; neutralization.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-HIV Agents / chemistry
  • Antibodies, Neutralizing / chemistry*
  • Biological Products / chemistry
  • CHO Cells
  • Cell Movement
  • Cell Proliferation
  • Cricetinae
  • Cricetulus
  • Drug Design*
  • False Positive Reactions
  • HIV Antibodies / chemistry*
  • HIV Infections / immunology*
  • HIV Infections / therapy
  • HIV-1
  • High-Throughput Screening Assays / methods*
  • Humans
  • Microfluidics
  • Microscopy, Fluorescence
  • Nanoparticles / chemistry
  • Nanotechnology / methods
  • Neutralization Tests
  • ROC Curve

Substances

  • Anti-HIV Agents
  • Antibodies, Neutralizing
  • Biological Products
  • HIV Antibodies