Structure of the O-specific polysaccharide from the deep-sea marine bacterium Idiomarina abyssalis КММ 227(T) containing a 2-O-sulfate-3-N-(4-hydroxybutanoyl)-3,6-dideoxy-d-glucose

Maxim S Kokoulin; Nadezhda A Komandrova; Anatoly I Kalinovskiy; Svetlana V Tomshich; Lyudmila A Romanenko; Victor V Vaskovsky

doi:10.1016/j.carres.2015.05.012

Structure of the O-specific polysaccharide from the deep-sea marine bacterium Idiomarina abyssalis КММ 227(T) containing a 2-O-sulfate-3-N-(4-hydroxybutanoyl)-3,6-dideoxy-d-glucose

Carbohydr Res. 2015 Sep 2:413:100-6. doi: 10.1016/j.carres.2015.05.012. Epub 2015 Jun 9.

Authors

Maxim S Kokoulin¹, Nadezhda A Komandrova², Anatoly I Kalinovskiy², Svetlana V Tomshich², Lyudmila A Romanenko², Victor V Vaskovsky³

Affiliations

¹ G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia. Electronic address: maxchem@mail.ru.
² G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.
³ G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia; Far Eastern Federal University, Vladivostok, Russia.

PMID: 26117228
DOI: 10.1016/j.carres.2015.05.012

Abstract

The O-specific polysaccharide was isolated from the lipopolysaccharide of type strain Idiomarina abyssalis КММ 227(T) and studied by sugar analysis, Smith degradation, and two-dimensional (1)H and (13)C NMR spectroscopy including (1)H,(1)H-TOCSY, (1)H,(1)H-COSY, (1)H,(1)H-ROESY, (1)H,(13)C-HSQC, (1)H,(13)C-HMBC, (1)H,(13)C-H2BC and (1)H,(13)C-HSQC-TOCSY experiments. The new structure of the O-specific polysaccharide of I. abyssalis КММ 227(T) containing 2-O-sulfate-3-N-(4-hydroxybutanoyl)-3,6-dideoxy-d-glucose was established.

Keywords: 2-O-Sulfate-3-N-(4-hydroxybutanoyl)-3,6-dideoxy-d-glucose; Extremophiles; Idiomarina abyssalis; Marine bacterium; O-Specific polysaccharide; Sulfate.

MeSH terms

Alteromonadaceae / chemistry*
Carbohydrate Sequence
Glucose / chemistry*
Molecular Sequence Data
O Antigens / chemistry*

Substances

O Antigens
Glucose