Haplotype analysis of TGF-β1 gene in a preeclamptic population of northern Mexico

Marisela Aguilar-Duran; Jaime Salvador-Moysén; Carlos Galaviz-Hernandez; Fernando Vázquez-Alaniz; Ada A Sandoval-Carrillo; Nadia Velázquez-Hernández; José M Salas-Pacheco

doi:10.1016/j.preghy.2013.07.007

Haplotype analysis of TGF-β1 gene in a preeclamptic population of northern Mexico

Pregnancy Hypertens. 2014 Jan;4(1):14-8. doi: 10.1016/j.preghy.2013.07.007. Epub 2013 Aug 9.

Authors

Marisela Aguilar-Duran¹, Jaime Salvador-Moysén¹, Carlos Galaviz-Hernandez², Fernando Vázquez-Alaniz³, Ada A Sandoval-Carrillo¹, Nadia Velázquez-Hernández¹, José M Salas-Pacheco⁴

Affiliations

¹ Instituto de Investigación Científica, Universidad Juárez del Estado de Durango, 34000 Durango, Mexico.
² Academia de Genómica, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional, IPN Unidad Durango, 34220 Durango, Mexico.
³ Hospital General de Durango, Secretaría de Salud Durango, 34000 Durango, Mexico.
⁴ Instituto de Investigación Científica, Universidad Juárez del Estado de Durango, 34000 Durango, Mexico. Electronic address: jsalas_pacheco@hotmail.com.

PMID: 26104248
DOI: 10.1016/j.preghy.2013.07.007

Abstract

Objective: To determine the frequencies of -800G/A (rs1800468), -509C/T (rs1800469) and 869T/C (rs1800470) polymorphisms and their haplotypes in the TGF-β1 gene and their association with preeclampsia in a population of northern México.

Design and methods: This case-control study involved 175 preeclamptic and 253 normoevolutive pregnant women. The polymorphisms were genotyped by real time PCR.

Results: The allele and genotype frequencies of polymorphisms showed no significant differences between cases and controls; the -800AA genotype had a very low frequency in cases (1%) and controls (0.4%). The TT genotype of the 869T/C polymorphism is a protective factor of severe preeclampsia (OR 0.56, 95% CI 0.32-0.98). The -509C/T and 869T/C polymorphisms were in linkage disequilibrium (D'=.537, p=.009). The most common haplotypes in case and control groups were -800G/-509C/869C, 34.95% and 37.24%, respectively. We found no increased risk of preeclampsia by haplotype.

Conclusions: Our results suggest that -800G/A, -509C/T and 869T/C polymorphisms of TGF-β1 gene or their haplotypes are not associated with preeclampsia and that only the TT genotype of 869T/C polymorphism is a protective factor of severe preeclampsia in a population of northern México.

Keywords: Haplotype; Polymorphism; Preeclampsia; TGF-β1.