Molecular cloning and comparative responses of Toll-like receptor 22 following ligands stimulation and parasitic infection in yellowtail (Seriola lalandi)

Fish Shellfish Immunol. 2015 Oct;46(2):323-33. doi: 10.1016/j.fsi.2015.06.020. Epub 2015 Jun 21.

Abstract

TLR22 is exclusively present in teleosts and amphibians and is expected to play the distinctive role in innate immunity. In this study, we cloned the full-length cDNA sequence of yellowtail (Seriola lalandi) TLR22 (SlTLR22). The complete cDNA sequence of SlTLR22 was 4208 bp and encodes a polypeptide of 961 amino acids. Analysis of the deduced amino acid sequence indicated that SlTLR22 has typical structural features of proteins belonging to the TLR family. These included 17 LRR domains (residues 91-633) and one C-terminal LRR domain (LRR-CT, residues 693-744) in the extracellular region, and a TIR domain (residues 800-943) in the cytoplasmic region. Comparison with homologous proteins showed that the deduced SlTLR22 has the highest sequence identity to turbot TLR22 (76%). Quantitative real-time PCR (qPCR) analysis demonstrated the constitutive expression of SlTLR22 mRNA in all examined tissues with higher levels in the head kidney, intestine, skin and spleen. Further, SlTLR22 expression was significantly up-regulated following TLR ligands injection with lipopolysaccharide (LPS), CpG ODN2006 and polyinosinic: polycytidylic acid (poly I:C) in spleen and liver. Amyloodinium ocellatum infection also induced a high expression of SlTLR22 in spleen, intestine, muscle, skin and gill, with maximum increases ranging from 1000 to 100 fold upon different ligands and organs. Finally, histological examination in gill tissue confirmed infection by the parasite and histopathological lesion was observed also in spleen and skin. These findings suggest a possible role of SlTLR22 in the immune responses to the infections of a broad range of pathogens that include DNA and RNA viruses and parasites.

Keywords: Expression modulation; TLR22; Yellowtail Seriola lalandi.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Dinoflagellida / physiology
  • Fish Diseases / immunology*
  • Fish Diseases / microbiology
  • Fish Diseases / parasitology
  • Fish Diseases / virology
  • Fish Proteins / genetics*
  • Fish Proteins / metabolism
  • Gene Expression Regulation*
  • Immunity, Innate*
  • Ligands
  • Lipopolysaccharides / pharmacology
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / pharmacology
  • Organ Specificity
  • Perciformes*
  • Phylogeny
  • Poly I-C / pharmacology
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sequence Alignment / veterinary
  • Toll-Like Receptors / genetics*
  • Toll-Like Receptors / metabolism

Substances

  • CpG ODN 2006
  • Fish Proteins
  • Ligands
  • Lipopolysaccharides
  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • Toll-Like Receptors
  • Poly I-C