A multi-layered vascular scaffold with symmetrical structure by bi-directional gradient electrospinning

Tong Wu; Chen Huang; Dawei Li; Anlin Yin; Wei Liu; Jing Wang; Jianfeng Chen; Hany Ei-Hamshary; Salem S Al-Deyab; Xiumei Mo

doi:10.1016/j.colsurfb.2015.05.048

A multi-layered vascular scaffold with symmetrical structure by bi-directional gradient electrospinning

Colloids Surf B Biointerfaces. 2015 Sep 1:133:179-88. doi: 10.1016/j.colsurfb.2015.05.048. Epub 2015 Jun 8.

Authors

Tong Wu¹, Chen Huang², Dawei Li², Anlin Yin¹, Wei Liu³, Jing Wang¹, Jianfeng Chen³, Hany Ei-Hamshary⁴, Salem S Al-Deyab⁵, Xiumei Mo⁶

Affiliations

¹ State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
² Engineering Research Center of Technical Textiles, Ministry of Education, College of Textiles, Donghua University, Shanghai 201620, China.
³ College of Material Science and Engineering, Donghua University, Shanghai 201620, China.
⁴ Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia; Department of Chemistry, Faculty of Science, Tanta University, Tanta 31527, Egypt.
⁵ Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
⁶ State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China; College of Material Science and Engineering, Donghua University, Shanghai 201620, China. Electronic address: xmm@dhu.edu.cn.

PMID: 26101818
DOI: 10.1016/j.colsurfb.2015.05.048

Abstract

Multi-layered scaffolds are advantageous in vascular tissue engineering, in consideration of better combination of biomechanics, biocompatibility and biodegradability than the scaffolds with single structure. In this study, a bi-directional gradient electrospinning method was developed to fabricate poly(l-lactide-co-caprolactone) (P(LLA-CL)), collagen and chitosan based tubular scaffold with multi-layered symmetrical structure. The multi-layered composite scaffold showed improved mechanical property and biocompatibility, in comparison to the blended scaffold using the same proportion of raw materials. Endothelialization on the multi-layered scaffold was accelerated owing to the bioactive surface made of pure natural materials. hSMCs growth showed the similar results because of its better biocompatibility. Additionally, fibers morphology change, pH value balance and long term mechanical support results showed that the gradient structure effectively improved biodegradability.

Keywords: Chitosan; Collagen; Electrospinning; Multi-layered vascular scaffolds; P(LLA-CL); Tissue engineering.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Blood Vessels*
Humans
Microscopy, Electron, Scanning
Tissue Scaffolds*