Preconcentration and detection of mercury with bioluminescent bioreporter E. coli ARL1

Appl Microbiol Biotechnol. 2015 Oct;99(20):8793-802. doi: 10.1007/s00253-015-6747-2. Epub 2015 Jun 23.

Abstract

Cell wall envelopes treated with sodium hydroxide and spray-dried were used as mercury sorbents. The sorbent having sorption capacity 17.7 ± 0.1 μmol/g determined was employed for preconcentration of mercury containing 1-10 ng/L. After preconcentration, bioavailable mercury was detected in samples of soil, stream, and tap water via induction of bioluminescence of E. coli ARL1. Iron and manganese at concentrations of tenth microgram per liter interfered bioluminescence detection of mercury. In tap water was detected semiquantitatively 0.127 ± 0.1 nmol/L by the induction of bioluminescence of E. coli ARL1 in medium with tryptone after preconcentration using a method of standard addition.

Keywords: Bioreporters; Biosorbents; Mercury detection; Mercury sorption.

MeSH terms

  • Biosensing Techniques / methods*
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism*
  • Luminescent Measurements
  • Mercury / analysis*
  • Soil / chemistry
  • Water / chemistry

Substances

  • Soil
  • Water
  • Mercury